| 日時: |
2005-06-17 14:00 - 15:00 |
| 場所: |
構造生物実験準備棟会議室 |
| 会議名: |
放射光セミナー「Structural basis of actin regulation by Formins and Rho GTPases」 |
| 講演者: |
Takanori Otomo Ph. D. (Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas) |
| 講演言語: |
日本語 |
| URL: |
http://pfwww.kek.jp/pf-seminar/ |
| アブストラクト: |
Diaphanous-related formins (DRFs) regulate dynamics of unbranched
actin filaments during cell contraction and cytokinesis. The conserved
formin homology 2 (FH2) domain nucleates actin filaments and remains
bound to the barbed end of the growing filament. I will discuss its
molecular mechanisms based on the crystal strucuture of the complex
between the FH2 domain and actin. DRFs are autoinhibited through
intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a
conserved N-terminal regulatory element. Autoinhibition is relieved
through binding of the GTPase RhoA to the N-terminal element. I will
discuss the mechanisms of Rho GTPase-mediated activation of the Formin
mDia1 though the crystal structure of the N-terminal element as well as
NMR and biochemical mapping experiments. |
