| DATE: |
2006-07-03 10:00 - 11:00 |
| PLACE: |
Rinko-shitsu 2F in 4-go-kan |
| TITLE: |
PF Seminar Allosteric Action and Protein Structural Relaxation Studied by Time-resolved X-ray Crystallography |
| SPEAKER: |
Dr. Vukica srajer (BioCARS, The University of Chicago) |
| LANGUAGE: |
English |
| URL: |
http://pfwww.kek.jp/pf-seminar/ |
| ABSTRACT: |
Time-resolved macromolecular crystallography is reaching a mature phase with demonstrated ability to detect small structural changes on ns and sub-ns time scale [1-6] and with important advances in the analysis of time-resolved crystallographic data, such as the use of Singular Value Decomposition (SVD) method to determine the structures of intermediates and elucidate the reaction mechanism [3-4]. We present results of ns time-resolved crystallographic studies of heme proteins: allosteric action in real time in cooperative dimeric hemoglobin and structural relaxation processes in myoglobin [1]. These pump-probe experiments were conducted at the BioCARS beamline 14-ID at the Advanced Photon Source (USA).
[1] Schmidt et al. PNAS 102 (33) 11704-11709 (2005);
[2] Ihee et al. PNAS 102 7145-7150 (2005);
[3] Rajagopal et al. Structure 13, 55-63 (2005);
[4] Schmidt et al. PNAS, 101, 4799-4804 (2004);
[5] Schotte et al. Science 300, 1944 (2003);
[6] srajer et al. Biochemistry 40, 13802 (2001).
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