| DATE: |
2006-07-03 11:00 - 12:00 |
| PLACE: |
Rinko-shitsu 2F in 4-go-kan |
| TITLE: |
PF Seminar Time-resolved crystallography - a technique coming of age? |
| SPEAKER: |
Dr. Reinhard Pahl (Center for Advanced Radiation Sources, The University of Chicago) |
| LANGUAGE: |
English |
| URL: |
http://pfwww.kek.jp/pf-seminar/ |
| ABSTRACT: |
Synchrotron radiation has profoundly influenced the field of macromolecular¡¡biology. Technological developments over the last decades have enabled new opportunities for rapid structure determination (structural genomics and proteomics) as well as the observation of short-lived structural intermediates during protein reactions. Utilizing the timing structure of the 3rd generation synchrotron radiation sources the laser pump - x-ray probe method was demonstrated in the early nineties, and developed to a mature technique with ~100ps time resolution in the following decade.
Meanwhile it has been successfully applied to numerous systems, e.g. the study of CO photo-dissociation and protein relaxation in myoglobin, the allosteric transition in hemoglobin (HbI), the photocycle of photoactive yellow protein (PYP) and catalytic reaction mechanism of various enzymes.
In this presentation I will provide a brief overview of the development of time-resolved crystallography, describe the present efforts at BioCARS Sector-14 at the Advanced Photon Source (APS), and discuss the challenges and opportunities for this technique at the next generation light sources.
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