We study collider phenomenology in the supersymmetric Standard Model with a certain type of non-universal gaugino masses at the gauge coupling unification scale, motivated by the little hierarchy problem. In this scenario, especially the wino mass is relatively large compared to the gluino mass at the unification scale, and the heavy wino can relax the fine-tuning of the higgsino mass parameter,so-called μ parameter. Besides, it will enhance the lightest Higgs boson mass due to the relatively large left-right mixing of top squarks through the renormalization group (RG) effect. Then 125GeV Higgs boson could be accomplished, even if the top squarks are lighter than 1TeV and the μ parameter is within a few hundreds GeV. The right-handed top squark tends to be lighter than the other sfermions due to the RG runnings, then we focus on the top squark search at the LHC. Since the top squark is almost right-handed and the higgsinos are nearly degenerate, 2b + MET channel is the most sensitive to this scenario. We figure out current and expected experimental bounds on the lightest top squark mass and model parameters at the gauge coupling unification scale.