Robotics
Preparation of Cryo Pin
Our robots usually handle the base CrystalCap Copper Magnetic 18mm and the Mounted Cryoloop sold by Hampton Research Co.
Please cut the Mounted Cryoloop at the position shown by a red arrow in the figure below. Then, please insert the cut Cryoloop in the base. If height of other pin is same as the height of the pin explained above, it may be able to used by our robots.
Loading pins into cassette
Storing sample information
CSV file includes the sample information.
The software "UGUIS" opens this CSV file and shows the sample information.
This CSV file is modified for automated data collection at the PF beamlines, based on the CSV file of SSRL. The software "UGUIS" can also read the SSRL CSV file.
Download: Template.csv (1kB)
Download: Example-SSRL.csv (8kB)
Download: Example-unipuck.csv (6kB)
| Row | Detail | Default value | |
| 1 | ContainerID | ID of cassette | (necessary) |
| 2 | Port | Port number in the sample cassette | (necessary) |
| 3 | CrystalID | ID of crystal | (necessary) |
| 4 | Protein | Name of protein | (necessary) |
| 5 | Directory | Directory name for storing data | - |
| 6 | Comment | Comment (Comma(,) cannot be used.) | - |
| 7 | Screening | 0: Manual screening, 1: Auto screening | 1 |
| 8 | Exp_result | Result of experiments | - |
| 9 | Run | Run number | 1 |
| 10 | Start | Number of start frame | 001 |
| 11 | Total_frame | Number of total frame | 180 |
| 12 | Start_omega | Start angle （deg.） | 0.0 |
| 13 | Snap_omega | Angles of samle rotation axis (snapshot) (blank separated)(deg.) |
0 30 60 90 |
| 14 | Osc_width | Oscillation angle (deg.) | 1.0 |
| 15 | Exp_time | Exposure time （sec.） | 1.0 (NE3A) 2.0 (BL-5A, NW12A) 5.0 (BL-17A) |
| 16 | Wavelength | Wavelength of X-ray （angstrom） | 1.000 |
| 17 | Max_resolution | Maximum resolution （angstrom） |
1.8 |
| 18 | Camera_height | Vertical offset of CCD detector from the center of direct beam (mm) | 0.0 |
| 19 | Hwidth | Width of horizontal slit (mm) | 0.2 (BL-5A, NW12A, NE3A) 0.05 (BL-17A) |
| 20 | Vwidth | Width of vertical slit (mm) | 0.2 (BL-5A, NW12A, NE3A) 0.05 (BL-17A) |
| 21 | Atten | Thickness of attenuator (mm) | 0 |
| 22 | Binning | Readout mode of CCD detector (0～2） 0：Full resolution、1：Software binning、2：Hardware binning |
0 (BL-1A, NE3A) 2 (BL-5A, 17A, NW12A) |
| 23 | Divergence | Beam divergence 0: Double slit, 1: Single slit |
1 |