Electroweak baryogenesis is a promising scenario to solve baryon asymmetry of the Universe, which is one of big mysteries of particle physics. I will discuss a scenario of electroweak baryogenesis in the two Higgs doublet model with quark flavor mixing. In general, off-diagonal components of quark Yukawa interactions with additional Higgs bosons are strongly constrained by the data for flavor changing neutral currents. However, top-charm quark mixing is not the case, so that a large off-diagonal element can be taken, which can contribute to generating baryon asymmetry of the universe. I will also discuss characteristic predictions for Kaon rare decays in the scenario of top-charm electroweak baryogenesis.