Photon Factory

KEK

BL-1A : Macromolecular Crystallography

Overview

Beamline group Group 4 (Protein Crystallography)
Technique
  • X-ray crystallography
Features
  • Rapid and accurate diffraction experiment using a small beam and pixel array detectors
  • Low background measurement under helium gas environment
  • Native SAD phasing using a long-wavelength X-ray(>2.7 Å)
Area of research
  • Structural biology
Contacts
  • Naohiro MATSUGAKI naohiro.matsugaki(at)kek.jp
  • Please change (at) to @ in the e-mail address.
Special notes

Performance

Source
  • 2.5GeV PF, Short Gap Undulator (SGU#01)
Optics
  • Monochromator (16.5 m):
    Cryo-cooled channel-cut Si (111)
  • Vertical Focusing Mirror (18.6 m):
    16 electrodes bimorph (600 mm (L) x 45 mm (W)), two stripes (Rh/SiO2) with 4.0 mrad glancing angle
  • Horizontal Focusing Mirror (19.2 m):
    12 electroldes bimorph (450 mm (L) x 45 mm (W)), two stripes (Rh/SiO2) with 4.0 mrad glancing angle
Energy range
  • 0.96 - 1.07 Å, 1.7 - 1.9 Å, 2.7 - 3.25 Å
Resolution (ΔE/E)
  • ~ 2 x 10-4
Beam size
  • 0.013 mm (H) x 0.013 mm (V)
Photon flux
  • 5 x 1010 photons/s (1.07 Å)
  • 1.1 x 1011 photons/s (2.7 Å)
Instruments
  • Cryocooling device: CryoIndusty Co. G2B-LT
  • Fluorescence detector: Amptek XR-100CR(Si-PIN)
  • Sample changer: PAM-HC (in-house developed)
Detectors
  • EIGER X 4M (x2) (DECTRIS Co.)
Remote / Mail-in
  • Available
Special notes

More information

References

  1. M. Hiraki et al., AIP Conf. Proc., 1741, 030029 (2016), doi:10.1063/1.4952852
  2. D. Liebschner et al., Acta Cryst., D72, 728-741 (2016), doi:10.1107/S2059798316005349
  3. S. Basu et al., IUCrJ, 6, 373-386 (2019), doi:10.1107/S2052252519002756

Publications

KEK-PF Publication Database (Publications of BL-1A)

Last updated: 2021-9-30